Heather Watson was one of three Britons to lose in the first round of French Open qualifying on Tuesday.
World number 156 Watson lost 6-3 5-7 6-1 to Croatia's 214th-ranked Antonia Ruzic.
In the men's tournament, Ryan Peniston fell 6-1 6-7 (5-7) 6-2 to American second seed JJ Wolf.
Oliver Crawford, who switched nationality to Britain from the United States in January, was beaten 6-7 (4-7) 6-4 6-2 by Li Tu of Australia.
The defeats mean Billy Harris is the sole Briton remaining in qualifying for the clay-court Grand Slam, which begins in Paris on Sunday.
Harris will face France's Manuel Guinard in the second round on Wednesday.