Riders at the Giro di Lombardia staged a protest which delayed the start of the race for five minutes as they sought to draw attention to safety concerns following a series of incidents.
Organisers completed the countdown and a starting flag was waved but the peloton remained stationary, creating confusion among spectators in Bergamo, Italy.
Belgium's Remco Evenepoel then addressed the crowd, saying "there's too many incidents happening with vehicles in the race" and highlighting the death of British cyclist Fin Tarling, who died in a collision with a vehicle at the Volta a Portugal in August.
"Unfortunately we lost the life of a brother and of a colleague of us, so we want to make this statement to show to the world that we cannot keep accepting the fact that guys are getting injured or literally dying in what we like the most to do," added double Olympic champion Evenepoel.
After five minutes had elapsed, a second starting flag was waved and the riders set off for Como.
"This week alone, there were multiple incidents of vehicles on race courses," said the CPA union, which represents professional cyclists, in a statement which was re-posted on Instagram by riders including Evenepoel and Great Britain's Tom Pidcock, external.
"This summer, a life was tragically lost to exactly this kind of failure. We will not stand for this any longer."
The CPA has called for cycling's governing body, the UCI, to help ensure race organisers "take responsibility" and "act" to improve safety standards.
Earlier this week, Thomas Gloag and Marcel Camprubi were struck by a police motorcycle travelling in the opposite direction during the Tre Valli Varesine race.
"Riders race at high speed with little protection, and we depend on the UCI and race organisers to secure the roads. That responsibility is not being met," the CPA statement added.
"We did not want to affect the race, only to show what riders can do when we stand as one.
"We love this sport. We respect the race organisers. We want to race hard and put on a show for our fans. However most importantly we want to come home safe to our loved ones."
The Giro di Lombardia is the last of the season's five Monuments, a set of long-standing, prestigious one-day races.
It has been won by Tadej Pogacar for the past five years but the Slovenian is not present at the race, which is also the penultimate event on this year's UCI World Tour calendar, after sustaining a season-ending injury at the Vuelta a Espana.

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